Novel fragrant starch-based films with limonene were successfully prepared. Biodegradable materials of natural origin were used and the process was relatively simple and inexpensive. The effect of limonene on physicochemical properties of starch-based films (moisture absorption, solubility in water, wettability, mechanical properties) were compared to glycerol plasticized system. Taking into consideration that the obtained materials could also exhibit bactericidal and fungicidal properties, the studies with Escherichia coli, Candida albicans and Aspergillus niger were performed. Such a material could potentially find application in food packaging (e.g. masking unpleasant odors, hydrophilic starch film would prevent food drying), or in agriculture (e.g. for seed encapsulated tapes).
A series of semicarbazones, thiosemicarbazones, 1,3,4-oxadiazoles/thiadiazoles bearing pyrazole scaffold were designed and synthesized. All the synthesized new compounds were characterized by 1H NMR, 13C NMR, MS and elemental analysis. The synthesized compounds were screened to probe their in vitro antimicrobial activity against bacteria and fungi species. The structure-activity relationship of the synthesized compounds was studied. The compounds displayed good to excellent potency against tested microorganisms. The in vitro antioxidant activities of the 1,3,4-oxadiazoles/thiadiazoles were evaluated by DPPH, hydroxyl and nitric oxide radical scavenging assay. Among the tested compounds, compound with chloro substitution showed good antioxidant potential.