A voltammetric epithelial sodium channels immunosensor using screen printed carbon electrode modified with reduced graphene oxide

Hartati, Y.; Yusup, S.; Fitrilawati, F.; Wyantuti, S.; Sofiatin, Y.; Gaffar, S.

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Current Chemistry Letters

ISSN 1927-730x (Online) - ISSN 1927-7296 (Print) Quarterly Publication Volume 9 Issue 4 pp. 151-160 , 2020

A voltammetric epithelial sodium channels immunosensor using screen printed carbon electrode modified with reduced graphene oxide Pages 151-160 Download PDF

Authors: Yeni Wahyuni Hartati, Sonya Fatimah Yusup, Fitrilawati Fitrilawati, Santhy Wyantuti, Yulia Sofiatin, Shabarni Gaffar

DOI: 10.5267/j.ccl.2020.2.001

Keywords: Epithelial sodium channels (ENaC), Anti-ENaC, Hypertension, Electrochemical immunosensor, Reduced graphene oxide

Abstract: Epithelial sodium channels (ENaC) is a protein that plays a role in maintaining sodium levels and can be used as a biomarker of hypertension. This study aims to develop an electrochemical immunosensor for detecting ENaC using a screen-printed carbon electrode (SPCE) which is modified with reduced graphene oxide (RGO). The performance of RGO enhanced to provide covalent bonds between the -COOH residues at RGO and the terminal -NH2 of anti-ENaC. While anti-ENaC and ENaC decrease the peak of ferric cyanides current because of the resistance effect. The optimum concentration of anti-ENaC was 1.0 µg/mL for 30 min incubation time. The various concentration of ENaC has a linear relationship with the decreasing of ferric cyanide peak current in the range of 0.01-1.5 ng/mL, and the limit of detection found at 0.198 ng/mL. The low-level exposure of ENaC warrants the realization of a point-of-care device for early detection of hypertension in urine samples.

How to cite this paper

 Hartati, Y., Yusup, S., Fitrilawati, F., Wyantuti, S., Sofiatin, Y & Gaffar, S. (2020). A voltammetric epithelial sodium channels immunosensor using screen printed carbon electrode modified with reduced graphene oxide.Current Chemistry Letters, 9(4), 151-160.

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