This paper presents an empirical investigation on how information and guidance offered by the bank influence on the barriers to adoption of mobile banking. Data were collected from a questionnaire from college students who attend Islamic Azad university in city of Tehran, Iran. Structural equation modeling of data from 425 respondents was used to test 5 hypotheses. The results show the information and instructions offered by bank have negative effect on 5 evaluated barriers including usage, value, risk, tradition and image to adoption of mobile banking.