Quality has always been one of the most important topics in the competitiveness of organizations’ service activities and it influences on brands, significantly. This paper presents an empirical investigation to study the effects of service quality on brand personality in hotel industry. The study uses two questionnaires, one for measuring brand personality by using Aaker’s questionnaire (Aaker, 1997) [Aaker, J. L. (1997). Dimensions of brand personality. Journal of Marketing Research, 34(3), 347-356.] and the other one for measuring service quality. The study chooses a hotel chain in city of Mashad, Iran named Sadr Hotel and examines the effects of five-brand personality on service quality by choosing a sample of 384 customers. Using structural equation modeling, the study has determined that all five factors could influence on service quality of the case study, positively.