Nowadays, smartphones are the leading dominant mobile communication devices, attributed to their multifunctional facilities and the mounting benefits provided by online platforms. From a business perspective, smartphones can be a practical substitute for conducting many business transactions online. Companies have transferred their traditional business models to e-business models employing new technologies. This paper aims to examine the entrepreneurs’ intentions to use smartphone applications in conducting business transactions as well as explore entrepreneurial opportunities in the Jordanian market. The study will use the quantitative method to examine the factors affecting Jordanian entrepreneurs’ intention to use Smartphone applications. A questionnaire was used to collect the required data from the study population. The respondents were selected using a random sampling method to obtain the needed data from entrepreneurs in Amman, Jordan. A total of 500 questionnaires were distributed, and 440 valid questionnaires were returned and included in the analysis. The revealed results show that ease of use, perceived security, and trust significantly influence entrepreneurs’ intentions to use Smartphone applications, while responsiveness shows no significant influence. The current study provides valuable information on the factors that nurture entrepreneurs' intentions to adopt smartphone applications in Jordan. Finally, the smartphone possesses high potential support for entrepreneurs in the business world. Entrepreneurs are advised to integrate smartphones more into business transactions to increase work efficiency benchmarks and boost their businesses.