Studying knowledge sharing behavior of lecturers at public universities in Ho Chi Minh City is an urgent issue. In the development and vastness of knowledge treasure, knowledge sharing actually becomes a way to reduce the difficulties and waste of time to learn, acquire the knowledge, thereby, helping public universities build a team of lecturers who have good knowledge and meet the job requirements for their development. Therefore, the author con-ducts a study to bring out the factors affecting activities of knowledge sharing behavior of lecturers at public universities in Ho Chi Minh City now. Based on the data collected, we use Cronbach’s Alpha, EFA and run regression model for knowing the impact levels of each independent variable on dependent variable of the knowledge sharing behavior of lecturers. Based on the findings, some recommendations are given for improving the knowledge sharing behavior of lecturers at public universities in Ho Chi Minh City.