This study aims to identify the level of Green Supply Chain practices and their Impact on Operational Performance of the Jordanian Private Hospitals. The Jordanian private hospital sector consists of 71 private hospitals comprising the study population. The convenience sample was used by distributing the research tool to 280 of the subjects in the study in Jordanian private hospitals. The responses received were 257 responses. Moreover, the analyses related to the research were performed using version 24 of SPSS and AMOS software, and test hypotheses structural equation modeling (SEM) was used. The results indicated that green supply chain practices have an impact on operational performance. Based on the study results, the researchers recommend managers to increase operational efficiency by Integrate sustainability parameters into product design requirements and assess them throughout the design process.