Job performance becomes one of crucial issues and challenges faced by the organizations and it is related to employees’ performance. In conjunction with the issue, this study investigates the determinants of job performance among female employees in Malaysia. Respondents involved in this study were composed of 320 female workers selected from manufacturing, education, health and hospitality sectors. The data was collected through self-administered questionnaires by using a five-point Likert scale. The study uses two common statistical analysis, including the descriptive and inferential statistics analyses by assisting statistical software, namely the Statistical Package for Social Science (SPSS-20). The outcome shows that motivation, organizational climate, and time management significantly influenced female employees' job performance. Also, the organizational climate was a determinant factor affecting female job performance in Malaysia. Thus, the finding of this study will assist female employees and employers in understanding the factors that could affect their performance in the organization.