High convergence level of strategic alignment between business and information technology helps companies enhance operational excellence. This research aims to identify strategic alignment maturity criteria level and their impacts on operational excellence in Jordanian industrial companies. The population of the study consists of all managers at top and middle management and a purposive sample is used to answer questionnaire items. In this survey, 288 valid responses are collected for analysis using SPSS (20) and smart PLS "V. 3". The study reveals that there is a significantly positive impact of strategic alignment maturity criteria (communication, competency-value Measurement, governance, partnership, scope & architecture and skills) on operational excellence. The most maturity criteria have a mid-level of maturity except scope & architecture and competency-value measurement criteria which maintain a low level. Thus, the study concluded that the researched companies have to recognize the business value of information technology (IT) investment and harmonizing IT architecture with business structure.