Polyester fibers are the most used in the manufacture of ropes for mooring systems and offshore operation, thus being constantly subjected to different situations. Such requests are implicated in a variety of load conditions, and their effects must be studied. This work presents data referring to an experimental study on the behavior of the quasi-static and dynamic stiffness of polyester yarns considering different mechanical levels of degradation and use. The study is performed with five different types of multifilament samples, these were extracted from a virgin spool and sub-ropes tested for tension and fatigue. The experimental procedure is carried out through an initial characterization where the linear density, the Yarn Break Load - YBL and the linear tenacity of the samples are determined. Continuing with the experimental tests, a procedure standardized by ISO 18962-2 is then carried out, consisting of three quasi-static stages and three dynamic stages, where the data acquired in the tests allow the determination of a dimensionless stiffness value. The results showed an increase in the quasi-static stiffness, tending to a plateau, and a linear increase in the dynamic stiffness, but with somewhat similar behavior between the samples. The results related to the total quasi-static stiffness also show that the specimens extracted from sub-ropes that underwent fatigue present greater total non-dimensionalized stiffness, this is indicative of the mechanical fatigue procedure as an improvement of the specimens, giving them greater stiffness, and consequently greater stresses rupture, a behavior that should be explored in future studies.