This paper presents an investigation to assess the relationship between female employees’ organizational citizenship behavior and job satisfaction within organization. A questionnaire is designed and distributed among employees who work in city of Qom, Iran and after analyzing the responses by SPSS software program, the relationships between variables are assessed by Pearson test after confirming the normality of the data using Kolmogrov-Smirnov test. The results of the studies reflect a strong relationship between citizen-organization behavior of female employees and job satisfaction. In addition, the survey examines six sub-hypotheses and confirms that there are positive and meaningful relationships between female employees’ custom, altruism, work consciousness, mutual coordination, fairness and courtesy on one side and job satisfaction on the other side.