The braking force of the tractor semi-trailer depends on many random factors and road parameters. Therefore, determining the braking force based on theoretical calculation or simulation is not accurate. This paper presents the method of setting up the braking force measurement system of the tractor semi-trailer on the road and constructing the braking dynamics model of the tractor semi-trailer to investigate the braking force using Matlab-Simulink software. The study results show that the average error between the simulation and experimental results of the tractor semi-trailer braking force is 9,81%.