This study investigated the relationships between Big-Five personality traits and mate preferences in Isfahan, Iran. The sample consisted of 225 single girls and boys that they were volunteer to complete the research measures. They completed of Neo- FFI-3 to evaluate personality traits and Iranian cultural preference questionnaire. To response to research question, data was examined in two steps. The first step was to examine the intercorrelate relations matrix among dependent and independent variables. The results showed extraversion is related to attractive appearance, educational level, no having sexy relations with opposite sex in single period, and no having addiction. Also attractive appearance and no having too close relations with each other family is correlated with neuroticism and conscientiousness. Also extraversion can predict attractive appearance and no having addiction preferences. Neuroticism can predict no having too close relations with mate families preference. Other variables, although contributing to the overall variance, were not significant predictors in our focused and cultural preferences.