This study develops a research framework to study the impact of green supply chains with three dimensions, namely green purchasing, green manufacturing, and green marketing to achieve competitive advantage with the existence of organizational ambidexterity as a mediating variable in Jordanian industrial companies. The study targeted the most important Jordanian industrial companies, which included 46 industrial companies out of 66 companies listed on the Amman Stock Exchange. The researchers personally administered 181 questionnaires, responses were analyzed using descriptive statistics, and the appropriate statistical methods were chosen to test the hypotheses of the study and reach its results. The findings indicate that all elements of green supply chain management, namely green purchasing, green manufacturing, and green marketing had a significant impact on competitive advantage. Also, green manufacturing and green marketing had a significant effect on organizational ambidexterity, but there was no significant impact for green purchasing on organizational ambidexterity, finally, the results of the study showed that organizational ambidexterity plays a significant mediating role in the relationship between green manufacturing, green marketing, and competitive advantage.