Bagasse and wheat bran are agrowaste and cause different environmental problems. These compounds contain highly valuable compounds that can be recycled by microorganisms. This work was carried out to evaluate the effect of fermentation by Bacillus subtilis on biological activity and chemical components of bagasse and wheat bran. The results demonstrated the antimicrobial activity of fermented wheat bran and bagasse against eight selected microbial pathogens exhibited high activity of fermented wheat bran and mix, fermented bagasse showed less antimicrobial activity. Fermented wheat bran and bagasse samples had the highest antioxidant activity values (16.45 and 14.94 %) in comparison with the unfermented samples (6.20 and 3.97 %) respectively. Concentration of protein, ash, oil, and carbohydrates in fermented wheat bran were 5.34 %, 7.30%, 2.36% and 10.6%, whereas in bagasse they were 2.45%, 1.68%, 1.51% and 3.25 respectively. The moisture contents in bagasse 73.1% were more than in wheat bran 63.88%.