This paper presents an empirical investigation to detect major barriers on developing nano technology in Islamic Azad University. The proposed study selects south Tehran branch as a pilot study, designs a questionnaire and distributes it among all 80 employees who work for this university. Cronbach alpha is calculated as 0.93, which is well above the minimum acceptable level. The study has executed the study in five different areas including financial resources, physical equipment, training and empowering human resources, organizational culture and outside organizational factors. The study has determined that physical equipment is number one priority followed by financial resources and training as well as empowering employees.