In reality, concrete structures are normally under various loadings, and results of different studies have shown that cracks in these structures and their materials, due to their nature as well as the loading type, do not develop along the crack plane (pure mode I); rather, they expand under mixed modes, making the crack growth studies under these modes a very important issue. In the crack growth phenomenon, the fracture toughness is a very effective parameter usually calculated by ENDB samples because they are easy to handle. In this study, several samples were made by changing the maximum aggregates size (dmax = 9.5, 12.5 & 19 mm) and the amount of hooked-end steel fibers (SF = 0.1, 0.3 & 0.5%), and tested under different loading modes (pure/mixed modes I and III) using the strain control jack device. According to the results, the lowest fracture toughness belonged to pure mode III, aggregates with dmax = 12.5 mm performed better in the self-compacting concrete reinforced with steel fiber, Also, the results show that the increasing trend of steel fibers does not have a positive effect on the fracture toughness performance.