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Synthesis and characterization of novel 4-(1-(4-(4-(4-aminophenyl)-1H-pyrazol-1-yl)-6-(4-(diethylamino)phenyl)-1,3,5-triazin-2-yl)-1H-pyrazol-4-yl)benzenamine fluorescent dye for protein binding
, Pages 1-12 Vikas S. Padalkar and N Sekar PDF (321 K) |
Abstract: Novel 4-(1-(4-(4-(4-aminophenyl)-1H-pyrazol-1-yl)-6-(4-(diethylamino)phenyl)-1,3,5-triazin-2-yl)-1H-pyrazol-4-yl)benzenamine fluorescent dye was synthesized by multistep synthesis from cyanuric chloride and phenyl acetonitrile. It has absorption at 360 nm with single emission at 497 nm having fairly good quantum yield (0.379). The intermediates and the dye were characterized by FT-IR, 1H NMR, 13C NMR and Mass spectral analysis. Its utility as biocompatible fluorescent dye was explained by conjugation with bovine serum albumin. The method is based on direct fluorescence detection of fluorophore labelled protein before and after conjugation. Purified fluorescent conjugates were subsequently analyzed by fluorimetry. The analysis showed that the tested conjugation reaction yielded fluorescent conjugates of the dye through carbodiimide chemistry. DOI: 10.5267/j.ccl.2011.12.001 Keywords: Bioconjugation, Bovine Serum Albumin 1,3,5-Triazine, Pyrazole, Fluorescence, |
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Nanosilica reinforced epoxy floor coating composites: preparation and thermophysical characterization
, Pages 13-20 Mir Mohammad Alavi Nikje , Mohammad Reza Khanmohammadi, Amir Bagheri Garmarudi and Moslem Haghshenas PDF (321 K) |
Abstract: In this study, flooring grade epoxy/nanoSiO2 nanocomposites were prepared by in-situ polymerization method. Nano silica was treated by coupling agent in order to surface treating and introducing of reactive functional groups to achieving adequate bonding between polar inorganic nano particles and epoxy organic polymer. γ-Aminopropyltriethoxysilane (Amino A-100) was used as an effective and commercially available coupling agent and nano silica treated in acetone media. SEM observations of cured samples revealed that the nano silica was completely dispersed into polymer matrix into nanoscale particles. Thermal and physical properties of prepared samples were investigated and data showed improvements in physical and mechanical properties of the flooring samples in comparison with unfilled resin. DOI: 10.5267/j.ccl.2011.12.002 Keywords: Reinforcement, Nanocomposite, Nanoparticle, Nano SiO2, Epoxy Flooring |
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Phosphomolybdic acid accelerated one-pot synthesis of 1-aryl-1H, 3H-thiazolo [3, 4-a] benzimidazoles
, Pages 21-26 Atul Chaskar PDF (136 K) |
Abstract: An efficient and practical, three-component one-pot condensation reaction between 1, 2-phenylenediamine, 2-mercaptoacetic acid and aromatic aldehydes in ionic liquids have been reported for the synthesis of 1-aryl-1H, 3H-thiazolo [3, 4-a] benzimidazoles derivatives in excellent yields. DOI: 10.5267/j.ccl.2011.12.003 Keywords: Phosphomolybdic acid, Ionic liquid, Multicomponent reaction, Benzimidazoles |
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Pyridinium Trifluoro Acetate Mediated Synthesis of 3,4-Dihydropyrimidin-2(1H)-ones and Tetrazolo[1,5-a]pyrimidine-6-carboxylates
, Pages 27-34 Chandran Raju, Madhaiyan Kalaipriy R. Uma, Radhakrishnan Sridhar and Seeram Ramakrishna PDF (136 K) |
Abstract: A simple and economic synthesis of 3,4-dihydropyrimidin-2(1H)-ones using pyridinium triflate as catalyst under microwave condition was attempted with an easy work-up protocol. Further tetrazolo [1,5-a] pyrimidine-6-carboxylates were synthesized by three-component coupling reaction of β-ketoesters with a mixture of aromatic aldehyde and 5-aminotetrazole. The products were well characterized with IR, NMR (1H and 13C NMR) and mass spectrometry. DOI: 10.5267/j.ccl.2011.12.004 Keywords: Dihydropyrimidinones, Tetrazolopyrimidines, Pyridinium Trifluoroacetate, Microwave Irradiation |
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Thermal investigation of Human Serum Albumin upon Interaction with Ytterbium (III)
, Pages 35-40 Mohammad Mirzaie, Lyla Barzegar, Gholamreza Rezaei Behbehani and Ali Akbar Saboury PDF (136 K) |
Abstract: In this paper complexation reaction between Yb3+ and Human serum albumin is examined using isothermal titration calorimetry (ITC). The extended solvation model was used to reproduce the enthalpies of HAS+Yb3+ interactions over the whole range of Yb3+ concentrations. The binding parameters recovered from this model were attributed to the structural change of HSA. The results show that Yb3+ ions bind to HSA with three equivalent affinity sites. It was found that in the high concentrations of the ytterbium ions, the HSA structure was destabilized. DOI: 10.5267/j.ccl.2011.12.005 Keywords: Isothermal Titration Calorimetry, Human Serum , Yb3+ Ion, Binding Parameters |
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Thermodynamic study on the interaction of Co2+ with Jack bean urease
, Pages 41-46 Lyla Barzegar, Gholamreza Rezaei Behbehani, Mohammad Mirzaie and Ali Taherkhani PDF (144 K) |
Abstract: The interaction of Jack Bean Urease with cobalt (II) ion was studied by Isothermal Titration Calorimetry (ITC) at 300 K and 310 K in 30 mM Tris buffer, pH=7. The stability of the enzyme increases due to its binding with cobalt ions. The extended solvation model was used to reproduce the heats of Co2++JBU interaction. It was found that there is a set of 12 equivalent and noninteracting binding sites for Co2+ ions. The association equilibrium constant and the molar enthalpy of binding are 4260.76M-1, -16.5 kJmol-1 at 300 K and 3438M-1, -16 kJmol-1 at 310 K, respectively. DOI: 10.5267/j.ccl.2011.12.006 Keywords: Isothermal Titration Calorimetry, Jack bean urease, Cobalt ion |
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Synthesis, photophysical properties of novel fluorescent metal complexes from 3-(1,3-benzoxazol-2-yl)naphthalen-2-ol, and their antimicrobial activities
, Pages 47-58 Kiran R. Phatangare, Amol B. Patil, Vikas S. Patil, Vikas S. Padalkar, Vinod D. Gupta, Prashant G. Umape and N. Sekar PDF (771 K) |
Abstract: A series of novel metal complexes containing metal atoms such as Zn, Co, Cu, Cd, Ni, Mg and Sn have been synthesized from 3-(1,3-benzoxazol-2-yl)naphthalen-2-ol. The synthesized ligand was well characterized by IR, 1H-NMR and mass spectrometry. The electrochemical properties were studied by cyclic voltammetric analysis. The synthesized complexes are fluorescent and absorb in the range of 317 to 323 nm while emit in the range of 371 to 416 nm with good quantum yield. All metal complexes show significant in vitro antibacterial activity against E. coli and S. aureus strains and in vitro antifungal activity against C. albicans and A. niger strains by using serial dilution method. The antibacterial activities were expressed as the minimum inhibitory concentration (MIC) in μg/mL. DOI: 10.5267/j.ccl.2011.12.007 Keywords: 3-(1,3-Benzoxazol-2-yl)naphthalen-2-ol, Metal complexes, Fluorescence, Antibacterial activity, Cyclic voltammetry |
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