Thiazolopyrimidnes are considered one of the most interesting classes in heterocyclic chemistry due to their pharmaceutical importance. Herein, we report the synthesis of some new heterocyclic compounds containing thiazolopyrimidine starting from compound (1) which was previously prepared in literature. The starting compound was allowed to react with different alkylating agents such as chloroacetone, chloroacetyl chloride, and phenacyl bromide to afford derivatives (2-4). Compound (5), benzylidene derivative, was obtained by the reaction of compound (2) with benzaldehyde while amino-dicarbonitrile compound (6) can be obtained by the reaction of compound (5) and malononitrile. Acetylation of amino group in compound (6) with chloroacetyl chloride led to formation of compound (7). Nucleophilic substitution of chlorine in compound (7) by aniline gave compound (8) which further subjected the Munich reaction to form compound (9). All new synthesized compounds were characterized using different elemental and spectral analysis.

Starting from 3-cyano-4,6-distyrylpyridin-2(1H)-thione (1), the compound N-(4-chlorophenyl)-2-((3-cyano-4,6-distyrylpyridin-2-yl)thio)acetamide (2) was prepared. Compound (2) underwent cyclization upon heating in ethanolic sodium ethoxide solution to give the corresponding cyclized form 3-amino-N-(4-chlorophenyl)-4,6-distyrylthieno[2,3-b]pyridine-2-carboxamide (3). The elemental analyses and spectroscopic data of compounds (2) and (3) are in agreement with their proposed structures. Their insecticidal activity against cowpea aphid, Aphis craccivora Koch, was studied. The results of insecticidal activity for compounds (2) and (3) against the nymphs and the adults of the tested insects exhibited that compounds (2) and (3) have a higher insecticidal activity than that of acetamiprid, a reference insecticide, after 24 h of treatment.

Past researches on 4-thiazolidinone nucleus have revealed the prominent potential of derivatives containing this nucleus to be developed as a potent therapeutic agent. Because of these biological activities, their structure-activity relationship has created an interest for medicinal chemists leading to the discovery of a number of lead molecules. This review highlights the routes for its synthesis and summarizes the past and recent studies on its biological activities to guide the medicinal chemists working on this nucleus in the development of clinically viable drugs.

A series of fourteen chalcone was synthesized via. Claisen–Schmidt condensation between substituted 2- hydroxyl acetonaphthones and substituted benzaldehyde in presence of tripotassium phosphate (K3PO4) catalyst. The reaction was carried out by conventional method using 2-methoxyethanol. The procedure is simple and efficient in terms of reaction time, easy workup and isolation of products and yields. In-vitro all these synthesized compounds were screened and evaluated for the cytotoxic and antimicrobial activity. It was found that these compounds had significant cytotoxic activity in comparison with standard 5-flurouracil. The compounds 3a, 3b, 3h, 3f and 3l were screened by MTT assay against liver cancer cell line-HepG2. Among these, the compound 3b and 3c showed LC50 values of 997.14 μM/ml and 284.13 μM/ml., respectively. The remaining compounds did not display the LC50 values. The compound 3l displayed the strongest cytotoxic activities with IC50 value of 91.85 μg/ml against liver cancer cell line. The Chalcone 3a, 3f, 3h and 3e demonstrated excellent antimicrobial activity and the remaining were moderately active against tested pathogens. The antimicrobial effects of all the tested compounds are due to the presence of pharmacological active substituent in the basic nucleus of Chalcones. Therefore, the present study leads to the development of new class of anticancer and antimicrobial inhibitory candidates.