The present study was designed to evaluate the amino acid profiles and anti-nutritional contents of six wild vegetables viz. Cardamine hirsuta, Melothria perpusilla, Cryptolepis sinensis, Persicaria chinensis, Lippia javanica and Polygonum perfoliatum from Assam, India. The total amino acid detected was found the highest in P. chinensis as 25.92 mg/g dry weight (DW) followed by P. perfoliatum (19.68 mg/g DW) and M. perpusilla (14.57 mg/g DW), and the lowest amino acid was observed in L. javanica (0.62 mg/g DW). However, the highest non-essential amino acids (NEAA) were detected in P. perfoliatum (3.89 mg/g DW). Among the NEAA, aspartic acid and glutamic acid were detected in all the six plant species which ranged from 0.01 to 0.33 mg/g DW and 0.02 to o.75 mg/g DW, respectively. In this study, the highest concentration of essential amino acids (EAA) was detected in C. hirsuta (1.72 mg/g DW) followed by M. perpusilla (0.95 mg/g DW) and P. chinensis (0.67 mg/g DW). Besides EAA and NEAA, some other amino acids such as phosphoserine, OH-proline, amino adipic acid, phosphoenolamine, and taurine are the most common which were detected. In the study, variable amounts of anti-nutritional contents were found and discussed herein.