This paper examines the relationship between entrepreneurial training, entrepreneurial culture and organizational performance. Accordingly, the study also investigates the moderating effect of gov-ernment funding on the relationship between entrepreneurial culture, entrepreneurial training and business performance among the public universities in Pakistan. The respondents include 415 heads of department out of 1100 identified in the study. Structural equation modeling using PLS 2.0 M3 reports significant relationship between entrepreneurial training and organizational performance. Likewise, entrepreneurial culture is also reported to be significantly important for boosting organizational performance. Importantly, the study reports significant moderation of government funding on the relationship between entrepreneurial culture and organizational performance. How-ever, no moderation is reported on the entrepreneurial training and organizational performance relationship. The findings of this study have enhanced the understanding regarding government funding in the prospect of improving the performance of the HEIs in Pakistan. The study recommends that the state government and higher education institutions in Pakistan allow room for entrepreneurial activities, and development of entrepreneurial principles and opportunities, and further encourage entrepreneurial practices through the development of public entrepreneurial orientation and entrepreneurial training on innovation to generate/create positive effects on the organizational performance of HEIs.