In the present world stiffeners are widely used to fulfill the requirement of high stiffness. Stiffened plates are used in most of the mechanical structures. In this paper free vibration analysis of eccentric and concentric stiffened isotropic plate with central stiffener and double stiffener has been studied and effect of various parameters such as boundary conditions, aspect ratio on non-dimensional frequency parameter of plate are investigated. Comparison of non-dimensional frequency parameter for eccentric and concentric isotropic stiffened plate with effect of number of stiffener at different mode shapes, aspect ratios and boundary conditions is studied using ANSYS parametric design language (APDL) code. A ten nodded element (SOLID 187) from ANSYS element library is used for the discretization of proposed stiffened isotropic plate. Convergence study of developed model with respect to the number of modes has been done and results are compared from the related available published literature. Non dimensional frequencies are higher for fully clamped boundary condition for eccentric and concentric stiffened isotropic plate in comparison to other boundary condition. It is also seen that non dimensional frequencies are increases in a moderate value for eccentric and concentric isotopic plate with double stiffener in comparison to the central stiffener.