This study intends to evaluate the impacts of implementing online marketing strategies on Iranian ecotourism industry. The study had eight hypotheses which were designed based on economic, sociological, and cultural aspects of Iranian ecotourism industry. The results indicate that the expansion of online marketing increased the number of foreign tourists who visited Iran’s natural tourist attractions, while it had no significant impact on the number of domestic tourists who visited these tourist attractions. The results also indicated that online marketing did not increase the amount of investment in Iranian ecotourism industry. Additionally, online ecotourism marketing did not have a significant influence on the total number of travels to Iran’s historical tourist attractions. Respondents believe implementing online marketing strategies in Iranian ecotourism industry helps Iran expand its cultural tourism in rural areas and it expands its hospitality industry; they also believe that online marketing helps to create more jobs in Iranian ecotourism industry and to improve working conditions in this industry.