A new series of Schiff bases derived from pyrazole-thiocarbohydrazide, namely (4a-d), were well-synthesized. The synthesis is carried out using monothiocarbohydrazone derivative (3), which was prepared via coupling of 5-chloro-pyrazole-4-carbaldehyde (1) with thiocarbohydrazide (2) in absolute ethanol that contains a catalytic quantity of acetic acid. The structures of newly synthesized compounds were fully clarified by various spectroscopic analyses (FT-IR, ¹H-NMR, ¹³C-NMR, and mass spectra) and elemental analysis. Also, the molecular docking was performed to investigate the binding interactions of the synthesized compounds (1, 3 and 4a-d) with COX-2 active site. The results revealed that most of them have robust hydrogen bonding networks and favorable binding energies compared to compound (4b). Understanding the anti-inflammatory behavior through understanding the specific interactions of these compounds with COX-2 will aid in the design and development of more effective inhibitors for therapeutic applications.

Quality and safety of drugs are essential for effective therapeutic performance. Impurities can compromise the quality and safety of drugs, and they can arise during various stages of the development, production, storage and even transportation process. Therefore, detecting and measuring the number of impurities with high accuracy in drugs is necessary to ensure the quality and safety of drugs and to reduce the risks associated with taking them. Detecting and measuring impurities in drugs require advanced analytical techniques. The review highpoints a variety of analytical chemistry techniques include spectrophotometric and chromatographic methods in addition to some electrochemistry methods that have been applied for determination of certain drugs such as Ciprofloxacin, Metronidazole, Hydroxychloroquine and Cefotaxime in their pure form, combined form with other drugs, combined form with degradation products, and in biological fluids.

An isocratic HPLC technique was exploited and validated for the quick simultaneous separation and measurement of cefotaxime and paracetamol in vials dosage forms, with a total analysis time of 3 minutes. The process of separation was carried out on a Thermo Scientific® Venusil XBPC18 (L) (5µm, 4.6x250 mm) using a mobile phase of ACN: distilled water (70:30, v/v) at the ambient temperature. The flow rate used in the experiment was 1 mL/min, and the highest level of absorption was determined by high-performance liquid chromatography with photodiode array detection (HPLC-PDA) employing a PDA detector set at a wavelength of 255 nm. The established retention times for cefotaxime and paracetamol were 1.79 and 2.97 minutes, respectively, suggesting reduced analysis duration. The observed limits of detection for ceftaxime and paracetamol were 4.2×10-5 and 1.2×10-5 µg/mL, respectively, indicating a significant level of sensitivity in the approach. The approach was subsequently verified in accordance with the requirements set out by the Food and Drug Administration (FDA) for the quantification of medicines in vial dosage form.

Pyrazolones are a class of heterocyclic compounds that contain a pyrazole ring fused to a ketone group. Recent scientific research has focused extensively on the potential anti-inflammatory properties of pyrazolone compounds due to their diverse pharmacological effects in alleviating inflammation and reducing fever. This motivated us to focus on the preparation of these derivatives in a simple and eco-friendly manner. A convenient new green methodology was modified for the preparation of 1-phenyl-3,5-pyrazolidinedione by the sonicated MCR of diethyl malonate, phenylhydrazine, and a catalytic amount imidazole as homogenous organic catalyst in water green solvent in a good yield. On the other hand, some of 4-arylidinepyrazolidinedione derivatives are prepared in the same manner via the treatment of a mixture of diethyl malonate, phenylhydrazine, aromatic aldehydes, and a catalytic amount of imidazole in an aqueous medium. Our target synthesized pyrazolidinediones were elucidated via elemental and several spectral analyses. Due to the importance of pyrazolidinediones in the field of treating inflammation and relieving pain, a number of prepared compounds were chosen to test their efficacy as anti-inflammatory agents using carrageenan-induced foot edema in rats and compare the results with indomethacin, the standard drug. We found that the majority of derivatives yield promising results spanning from good to wonderful, so derivatives (15k, 15b, 15h, 15a, and 15j) yield the best results while derivative (15i) yields an average result. As for the derivative (15f), it yields the lowest results compared to the standard drug. This is due to the difference in the structural composition of these derivatives, which increases the likelihood of their use as anti-inflammatory derivatives.

2-Indolinone nucleus is considered one of a promising heterocyclic core in medicinal chemistry that showed numerous range of activity among which antimicrobial, antioxidant, antiviral, antitubercular and anticancer activities. Cancer targeting is still an issue so there is a need for developing new agents that inhibit cancer growth without or low effect on normal body cells. Some derivatives of indolin-2-one are known to be a critical structure in some inhibitors of receptor tyrosine kinases (RTKs); a cancer target therapy, for example, Sunitinib. Herein in this review we focus on 2-indolinone derivatives as RTKs inhibitors as cancer targeting therapy.

This manuscript reports the preparation and toxicological efficacy testing of nine oxoimidazolidine and cyanoguanidine compounds (3a, 3b, 3c, 3d, 3e, 3f, 3g 4a, and 4b) against cowpea aphid, Aphis craccivora Koch. Bioefficacy data revealed that the tested compounds exhibited a range of toxicological activities against these insects, with compound 3b being the most toxic and compound 4a being the least effective. The LC50 value of compound 3b was 1.72 ppm for adults and 0.02 ppm against nymphs of the cowpea aphid, while the LC₅₀ value of compound 4a was 72.51 ppm for adults and 18.02 ppm against nymphs. The manuscript also presents the structure-activity relationships of these compounds. These results provide valuable insights into the development of effective pest control agents for the management of such insects.

The food industry consumes large amounts of water although there is an increasing demand for water and a rapid decrease in the level of natural water resources. Wastewater resulting from food industries needs to be assessed for their compliance to standards. In this study, wastewater treatment steps from the food industry were investigated for accurate assessment of wastewater loading by analyzing parameters of the concentration of compounds present in the effluents. The results revealed that the parameters of treated wastewater were as follow, electrical conductivity 2931 μs/cm, total suspended solids 100 mg/L, biochemical oxygen demand 90 mg/L, chemical oxygen demand 250 mg/L, total phosphorus 7.9 mg/L, and total nitrogen 70 mg/L. This exerts a huge load on the biological treatment unit. Thus, this study offers an understanding and support in selecting appropriate treatment for industrial wastewater to obtain an effluent suitable in compliance with standards of the environmental quality.

Herbicides used on a regular basis could endanger non-target species like earthworms. The aim of this work was to test the toxic effect of atrazine and metribuzin on Aporrectodea caliginosa by filter paper contact and soil mixing techniques. Atrazine had the highest intrinsic toxicity to earthworms, with LC50 of 0.026 µg mL-1 after 72 hr of treatment. While the LC50 of metribuzin was 0.063 µg mL-1 after 72 hr by filter paper contact test. LC50 was reduced from 11.121 to 3.118 and 164.824 to 19.113 μg g soil-1 in clay soil, from 32.221 to 17.33 and 324.141 to 41.028 μg g soil-1 in soil (1:1) and from 41.234 to 30.804 and 462.255 to 70.902 μg g soil-1 in sandy clay loam soil of atrazine and metribuzin after 5 and 10 day. Generally, atrazine is more toxic than metribuzin in both tests.

Increasing the yield and nutritional value of faba beans is one of the main objectives of the common bean cultivar improvement programs due to its contribution to terminate protein deficiency malnutrition in developing countries and raising the income level of smallholder farmers. In this study, the technological quality of bean seeds from twenty-four genotypes were evaluated (twenty germplasm and four improved cultivars (Misr 1, Giza 429, Giza 716, Giza 843). The results showed a significant diversity in the Egyptian bean germplasm in their quality traits compared to the control cultivars and indicated that the genotypes had a high hydration coefficient that ranged between (98.6-112.4%). While most of the genotypes for cotyledons to hull ratio met the commercial criteria that ranged between (7.41-6.41%). Twenty genotypes had the highest total soluble solids (6.90-11.78%) and were superior to the control cultivars. The seeds chemical composition analysis showed that the genotypes differed in their composition such as protein (26.65 to 30.72 %.), carbohydrate (58.00 to 62.25%), tannin (70.67 to 157.45%), phenols (35.23-51.02 %), and moisture (9.15 to 10.45%).

In developing countries, it still suffers from endless problems regarding wastewater treatment and the problem of choosing the appropriate treatment system due to lack of proper technology and weak economy. This study highlights the maturity oxidation pond (stabilization) of small communities as an effective, low-cost and simple post-treatment technique for treating wastewater before discharging into an aquatic ecosystem. The Rashid plant was cited as a treatment plant in the city of Rashid-ElBeheira Governorate - Egypt as a model plant for applying this technology in treatment of wastewater. This work also includes the studying of the relationship between climatic conditions, physicochemical parameters and biomass of microorganisms to evaluate the efficiency of its performance.