Knowledge plays an essential role on creating value added business units in today’s competitive environment. In this paper, we perform a study to investigate the relationship between knowledge management and organization culture in an Iranian PVC industry. The proposed study selects a sample of 65 people and distributes a questionnaire among them. The study investigates the relationship between knowledge management and four organizational cultures’ components including mission, consistency, involvement and adaptability. Using Denison model and implementation of Pearson correlation ratio as well as stepwise regression techniques, we analyze four hypotheses of the survey. The results of the study confirm that there are strong relationships between knowledge management from one side and mission, consistency, involvement and adaptability.