This study aims to examine total quality management, regulation, and blood production relationships of blood banks in Uganda. A structured questionnaire was used to collect data from 146 randomly selected respondents. The model was validated using Smart PLS-SEM analysis. The findings indicate that both total quality management and regulation have a significant and positive influence on blood production, accounting for a 17.8% variation at a 95% confidence interval. Regulation exhibited no mediation effect in the relationship between total quality management and blood production. Total quality management and regulation are essential factors enhancing blood production. Prioritizing total quality management practices in areas such as determining and meeting customer needs; customer and employee satisfaction surveys; and market research can optimize blood production. This study contributes to the blood bank management knowledge body and identifies areas to support blood production. Blood bank managers can apply these insights to improve operational performance.