This paper presents an empirical investigation to study the effects of organizational culture and information technology on knowledge sharing in national foundation of computer games (NFCG) located in city of Tehran, Iran. The proposed study designs a questionnaire in Likert scale, distributes it among 55 selected people who work for NFCG, which consists of three categories of organizational culture, information technology and knowledge sharing and Cronbach alphas are calculated as 0.869, 0.707 and 0.773, respectively. Using ANOVA test, the study has determined a positive and meaningful relationship between organizational cultures and knowledge sharing. However, the study has not determined any positive and meaningful relationship between information technology and knowledge sharing. Furthermore, while the results of the study have not indicated any meaningful relationships between trust, employee communication on one side and knowledge sharing on the other side, there were positive and meaningful relationships between reward system and organizational structure on one side and knowledge sharing on the other side.