Eco-friendly business practices range from energy efficiency to sustainable management; therefore, the implementation of these practices not only has the potential to mitigate environmental impacts, but also to improve the long-term competitiveness and sustainability of companies. In this study we analyze eco-friendly business practices and their impact on environmental sustainability in companies in a region of the Peruvian Amazon. A quantitative, non-experimental approach was considered, with an explanatory design; for which, a sample size of 200 companies was considered, which were randomly selected, to which two instruments were applied, the first with 7 indicators and the second with 3, valid, with an alpha of 0.720 and 0.670 respectively, assessing structural equation modeling. The results show that eco-friendly business practices are oriented towards sustainability (C.R. = 19.280), as well as preventive and eco-efficient practices (C.R. = 5.023, C.R.= 14.185); these findings can serve as a basis for the creation or improvement of public policies that promote eco-friendly business practices, encouraging companies to adopt measures that favor environmental sustainability. Therefore, eco-friendly business practices have a positive impact on environmental sustainability in companies in a region of the Peruvian Amazon; in addition, preventive practices and eco-efficient practices have a positive impact on environmental sustainability in companies in a region of the Peruvian Amazon.