This study examines gender differences in attitudes and intentions to adopt artificial intelligence among startup professionals. Utilizing a survey methodology encompassing responses from male and female participants, key constructs including attitude, perceived ease of use, perceived usefulness, and intention to use were analyzed through a comparative lens. The results reveal nuanced disparities between male and female perspectives on AI adoption. While minor differences were observed in the influence of attitude and perceived ease of use on adoption intentions, a significant gender gap emerged in the perception of how ease of use impacts perceived usefulness. These findings underscore the importance of recognizing gender dynamics in shaping attitudes and intentions towards AI adoption, highlighting the need for gender-inclusive strategies in fostering technology adoption among startups. This study contributes to the understanding of gender-specific considerations in AI adoption processes and offers insights for policymakers and industry stakeholders seeking to promote equitable and inclusive technological advancement.